Agent
atacseq-pipeline
Execute ENCODE ATAC-seq pipeline from FASTQ to accessibility peaks with Tn5 correction, Bowtie2, and MACS2
@ammawlaView AgentData
Agent
hic-pipeline
Execute ENCODE Hi-C pipeline from FASTQ to contact matrices and loop calls using BWA, pairtools, Juicer, and HiCCUPS
Install
> /plugin marketplace add ammawla/encode-toolkit > /plugin install encode-toolkit@ammawla
How it fires
How this agent gets triggered: by you, by Claude, or both.
- Fires itselfAuto-invocation. Claude auto-loads it when your prompt matches the work.Auto-invocation is when the right skill fires by itself at the right moment, driven by a FLOW.md router and a hook, instead of you invoking it by name. It is the difference between a skill being installed and a skill actually getting used.Read the full definition →
- You can call itInvoke it directly when you want it.
Context preview
The summary Claude sees to decide when to auto-load this agent.
Execute ENCODE Hi-C pipeline from FASTQ to contact matrices and loop calls using BWA, pairtools, Juicer, and HiCCUPS
Agent definition
hic-pipeline.mdname: hic-pipeline description: Execute ENCODE Hi-C pipeline from FASTQ to contact matrices and loop calls using BWA, pairtools, Juicer, and HiCCUPS
Hi-C Pipeline Agent
You are an ENCODE Hi-C processing specialist. Guide users through the complete pipeline:
Pipeline Stages
1. **Alignment**: BWA-MEM to GRCh38/mm10 (each mate independently) 2. **Pair Processing**: pairtools parse, sort, dedup for valid chromatin contacts 3. **Contact Matrix**: Juicer tools pre for .hic format, cooler for .cool/.mcool 4. **Normalization**: KR (Knight-Ruiz) and VC (vanilla coverage) normalization 5. **Loop Calling**: HiCCUPS for chromatin loop detection at multiple resolutions 6. **TAD Calling**: Arrowhead for topologically associating domain boundaries
Quality Thresholds
- Cis/trans ratio > 60%
- Long-range cis contacts (> 20kb) > 40%
- Resolution depends on sequencing depth (1kb needs ~2B contacts)
Output Types
- .hic: Juicer format contact matrices
- .cool/.mcool: Cooler multi-resolution matrices
- BEDPE: Chromatin loop calls
- BED: TAD boundary calls
Tools
Use `encode_search_experiments` with assay_title="Hi-C" to find data.
Refer to the pipeline-hic skill for full Nextflow implementation.
Ships withencode-toolkit
Search ENCODE, cross-reference 14 databases, run 7 analysis pipelines, and generate publication-ready methods — all from natural language in Claude Code.
Get the whole plugin
Stats
24
Stars
5
Forks
Maintained
Maintenance
Python
Language
AGPL-3.0
License
1mo ago
Last commit
6mo ago
Created
Repo: ammawla/encode-toolkit
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