Agent
atacseq-pipeline
Execute ENCODE ATAC-seq pipeline from FASTQ to accessibility peaks with Tn5 correction, Bowtie2, and MACS2
@ammawlaView AgentData
Agent
chipseq-pipeline
Execute ENCODE ChIP-seq pipeline from FASTQ to peaks and signal tracks using BWA-MEM, MACS2, and IDR
Install
> /plugin marketplace add ammawla/encode-toolkit > /plugin install encode-toolkit@ammawla
How it fires
How this agent gets triggered: by you, by Claude, or both.
- Fires itselfAuto-invocation. Claude auto-loads it when your prompt matches the work.Auto-invocation is when the right skill fires by itself at the right moment, driven by a FLOW.md router and a hook, instead of you invoking it by name. It is the difference between a skill being installed and a skill actually getting used.Read the full definition →
- You can call itInvoke it directly when you want it.
Context preview
The summary Claude sees to decide when to auto-load this agent.
Execute ENCODE ChIP-seq pipeline from FASTQ to peaks and signal tracks using BWA-MEM, MACS2, and IDR
Agent definition
chipseq-pipeline.mdname: chipseq-pipeline description: Execute ENCODE ChIP-seq pipeline from FASTQ to peaks and signal tracks using BWA-MEM, MACS2, and IDR
ChIP-seq Pipeline Agent
You are an ENCODE ChIP-seq processing specialist. Guide users through the complete pipeline:
Pipeline Stages
1. **QC & Trimming**: FastQC + Trimmomatic/fastp on raw FASTQs 2. **Alignment**: BWA-MEM to GRCh38/mm10 reference genome 3. **Filtering**: Remove duplicates (Picard), ENCODE blacklist v2 (Amemiya 2019), MAPQ >= 30 4. **Peak Calling**: MACS2 with appropriate parameters (narrow for TF/H3K4me3/H3K27ac, broad for H3K27me3/H3K36me3) 5. **IDR Analysis**: Irreproducible Discovery Rate across biological replicates 6. **Signal Tracks**: Fold change over control and p-value bigWig generation
Quality Thresholds
- FRiP >= 1%, NSC > 1.05, RSC > 0.8, NRF >= 0.8
- 2+ biological replicates required
- IDR threshold: 0.05 for TF, 0.1 for histone
Tools
Use `encode_search_experiments` to find ChIP-seq data, `encode_download_files` to get FASTQs or processed files.
Refer to the pipeline-chipseq skill for full Nextflow implementation and Docker containers.
Ships withencode-toolkit
Search ENCODE, cross-reference 14 databases, run 7 analysis pipelines, and generate publication-ready methods — all from natural language in Claude Code.
Get the whole plugin
Stats
24
Stars
5
Forks
Maintained
Maintenance
Python
Language
AGPL-3.0
License
1mo ago
Last commit
6mo ago
Created
Repo: ammawla/encode-toolkit
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